Membrana de Quitosana-Insulina-Tripolifosfato para Liberação Controlada de Fármacos: Efeito da Reticulação
Preparation and Characterization of Chitosan-Insulin-Tripolyphosphate Membrane for Controlled Drug Release: Effect of Cross Linking Agent
Author(s):
Holanda Adriana Lima¹, Fook Marcus Vinícius Lia², Swarnakar Ramdayal³*
ABSTRACT
Diabetes Mellitus (DM) encompasses a group of metabolic disorders characterized by chronic hyperglycemia resulting from defects in insulin secretion and/or action. Insulin therapy remains the preferred treatment for DM, typically involving daily subcutaneous insulin injections to manage blood glucose levels. Chitosan, a biomaterial widely studied for biomedicine, shows promise for controlled drug release. Its release rate can be modulated by Sodium Tripolyphosphate (TPP), an ionic cross-linker. This study aimed to develop and evaluate chitosan membranes, both with and without TPP cross-linking, for controlled insulin release, offering a potential alternative to injectable administration.
The developed membranes underwent characterization using Fourier Transform Infrared spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Energy Dispersive X-ray Spectroscopy (EDX), High Performance Liquid Chromatography (HPLC), and Macrophage Cell Viability Evaluation (MTT assay). FTIR analysis confirmed interactions between chitosan, tripolyphosphate, and insulin. EDX identified the chemical elements present in the chitosan, insulin, and sodium tripolyphosphate membranes. SEM revealed morphological changes in the insulin-containing membrane, showing granular particles of varying sizes compared to pure chitosan. HPLC confirmed the presence of insulin and demonstrated its release from the chitosan membrane, even when cross-linked with TPP, albeit at a slower rate. The crosslinking agent effectively controlled the insulin release rate. The MTT assay confirmed the biocompatibility of the prepared membranes through macrophage cell viability. These findings suggest that the developed membranes hold potential as a biomaterial for controlled insulin release systems.
Disponível em: https://www.scirp.org/pdf/JBNB_2014093015335723.pdf